Protein Purification Reagents and Kits
Cayman Chemical GlutathIon ethyl ester 100mg
A cell-permeable derivative of GSH; increases mitochondrial GSH levels in C38 and IB3-1 epithelial lung cystic fibrosis cells at 10 mM; decreases reactive oxygen species (ROS) levels and TNF-α-induced IL-8 secretion in IB3-1 cells at 10 mM; increases mitochondrial complex I function and reduces depolarization of the mitochondrial membrane potential in IB3-1 cells at 10 mM and 1mM, respectively; decreases lipid oxidation and ferroptosis in cystine-deficient PANC-1, AsPC-1, BxPC-3, and S2-013 pancreatic cancer cells; increases the viability of irradiated human lymphoid cells at 5 mM
Cayman Chemical MK2 InhIbItr III 1mg
A potent, cell-permeable inhibitor of MK2 (IC50 = 8.5 nM); less potently blocks MK3 and MK5 (IC50s = 210 and 81 nM, respectively) and is weak or inactive against several other kinases, including other p38 MAP kinase targets; prevents LPS-induced synthesis of TNF-α in human monocyte-like U937 cells (IC50 = 4.4 µM)
STEMCELL Technologies EasySep™ HLA Chimerism Whole Blood CD8 Positive Selection Kit, For processing: 60 mL whole blood processed
The EasySep™ HLA Chimerism Whole Blood CD8 Positive Selection Kit is designed to isolate CD8+ cells from fresh whole blood or buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD8 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD8 antigen is expressed on cytotoxic T cells and weakly on a subset of NK cells.This product replaces the EasySep™ Human Whole Blood CD8 Positive Selection Kit (Catalog #18083) for even faster cell isolations.
STEMCELL Technologies EasySep™ Human EpCAM Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Human EpCAM Positive Selection Kit II is designed to isolate EpCAM+ cells from fresh or previously frozen human mammary epithelial cell preparations by immunomagnetic positive selection. Desired cells are targeted with antibody complexes recognizing EpCAM and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The EpCAM+ fraction contains luminal cells, luminal-restricted, and bipotential progenitor cells.This product replaces the EasySep™ Human EpCAM Positive Selection Kit (Catalog #18356).
Neuromics, Inc 10x Buffer BXT, Strep-Tacting XT Elution Buffer, 25 ml contains a Biotin concentration for competitve displacement of purified recombinant proteins.
Buffer BXT is a Strep-TactinXT Elution Buffer with Biotin. It contains a Biotin concentration for competitive displacement of purified recombinant Strep-tagII or Twin-Strep-tag fusion proteins from immobilized Strep-TactinXT.Buffer BXT is also recommended for efficient elution in combination with Strep-TactinXT and MagStrep "type3" XT beads (2-4090-002).
STEMCELL Technologies EasySep™ Non-Human Primate CD4+ T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Non-Human Primate CD4+ T Cell Isolation Kit is designed to isolate CD4+ T cells from fresh peripheral blood mononuclear cells by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.This kit has been verified for use with rhesus and cynomolgus macaques.
Norgen Biotek Corp AAV Purification Mini Kit
Recombinant adeno-associated virus (AAV) vectors are highly promising tools for both in vitro and in vivo gene transfer. Norgen’s AAV Purification Mini Kit provides a fast and simple procedure for concentrating and purifying AAV vectors from cell lysate and cell culture media. Purification is based on spin column chromatography using Norgen’s proprietary resin as an ion exchanger. Contaminating cellular debris is largely removed from the sample via a precipitation and centrifugation step, while contaminating DNA and RNA is reduced using enzymatic digestion. AAV vector purified in this manner is highly active for use in in vitro transduction experiments, and is eluted into a small volume (200 µL).
STEMCELL Technologies EasySep™ Direct HLA Crossmatch B Cell Isolation Kit, For processing: 100 mL whole blood processed
The EasySep™ Direct HLA Crossmatch B Cell Isolation Kit can be used to isolate highly purified B cells directly from human whole blood, spleen, or lymph nodes by immunomagnetic negative selection. This cell isolation kit targets non-B cells for removal with antibodies recognizing specific surface markers. Unwanted cells are labeled with antibodies and EasySep™ Direct RapidSpheres™, and separated using an EasySep™ magnet. Desired cells are simply collected into a new tube and and are immediately available for downstream applications such as flow cytometry crossmatch.
Bioassay Systems EnzyChrom™ Neuraminidase Assay Kit. For quantitative determination of neuraminidase activity and screen for neuraminidase inhibitor.
For quantitative determination of neuraminidase activity and screen for neuraminidase inhibitor. Key Features: Sensitive and accurate. Linear detection range at 37°C in 96-well plate: 0.1 to 10 U/L for colorimetric assays and 0.01 to 2 U/L for fluorimetric assays. Simple and convenient. Homogeneous assay requiring only two absorbance measurements. Assay can be completed in 60 min. High-throughput. Can be readily automated as a high-throughput 96-well plate assay to screen thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: OD, FL: 1, 0.01U/L.
CUBE BIOTECH PureCube 100 Ni-NTA Agarose; 250 ml
250 ml of Ni-INDIGO MagBeads for His-tag-purification, 50% slurry
Cayman Chemical MK2 InhIbItr IV 10mg
A highly selective, non-ATP competitive inhibitor of p38/mitogen-activated protein kinase-activated protein kinase 2 (IC50 = 0.11 μM); inhibits TNF-α (IC50 = 4.4 μM) and IL-6 (IC50 = 5.2 μM) secretion from the human THP-1 acute monocytic leukemia cell line
STEMCELL Technologies EasySepTM Direct Human B Cel
EasySep(TM) kit with RoboSep(TM) buffer and RoboSep(TM) filter tips - 100 mL whole blood processed
Biolegend MojoSortTM Human Pan Monocyte
MojoSort™ Human Pan Monocyte Isolation Kit; Reactivity: Human; Apps: Cell Sep - Neg; Size: 20 tests